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  4. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells
 
research article

Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells

Wulhfard, Sarah  
•
Baldi, Lucia  
•
Hacker, David L.  
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2010
Journal of biotechnology

Valproic acid (VPA) is a small molecule that inhibits histone deacetylase activity. Here we report that VPA increases recombinant mRNA and protein levels in transiently transfected CHO DG44 cells. In the presence of VPA, transient recombinant antibody yields of up to 40 mg/L were achieved in simple batch cultures. The steady-state levels of the IgG light and heavy chain mRNAs were nearly 10 times higher than in the untreated control transfection even though the level of transfected plasmid DNA was the same in the presence or absence of VPA. The combination of VPA treatment and incubation of the transfected cells in mildly hypothermic conditions resulted in recombinant antibody yields of over 90 mg/L by 6 days post-transfection in batch cultures. The results demonstrated that the treatment of transfected CHO DG44 cells with VPA is a cost-effective strategy for enhancing transient gene expression by increasing the transgene mRNA levels.

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Type
research article
DOI
10.1016/j.jbiotec.2010.05.003
Web of Science ID

WOS:000280395100006

PubMed ID

20510314

Author(s)
Wulhfard, Sarah  
Baldi, Lucia  
Hacker, David L.  
Wurm, Florian  
Date Issued

2010

Publisher

Elsevier

Published in
Journal of biotechnology
Volume

148

Issue

2-3

Start page

128

End page

32

Subjects

Valproic acid

•

Histone deacetylase inhibitor

•

Transient gene expression

•

Transgene mRNA

•

CHO cells

•

Orbital shaking

•

Polyethylenimine

•

Low Culture Temperature

•

Gene-Expression

•

Mammalian-Cells

•

In-Vivo

•

Transcription Level

•

Sodium-Butyrate

•

Cho-Cells

•

Productivity

•

Inhibitors

•

Growth

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LBTC  
Available on Infoscience
August 27, 2010
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/52514
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