Use of Force Spectroscopy to Investigate the Adhesion of Living Adherent Cells

The use of force spectroscopy to study the adhesion of living fibroblasts to their culture substrate was investigated. Both primary fibroblasts (PEMF) and a continuous cell line (3T3) were studied on quartz surfaces. Using a fibronectin- coated AFM cantilever, it was possible to detach a large proportion of the 3T3 cells from the quartz surfaces. Their adhesion to the quartz surface and the effects of topography on this adhesion could be quantified. Three parameters characteristic of the adhesion were measured: the maximum force of detachment, the work of adhesion, and the distance of detachment. Few PEMF cells were detached under the same experimental conditions. The potential and limitations of this method in measuring cell/surface interactions for adherent cells are discussed.


Published in:
Langmuir, 26, 11, 8180–8186
Year:
2010
Publisher:
American Chemical Society
ISSN:
0743-7463
Laboratories:


Note: The status of this file is: EPFL only


 Record created 2010-07-13, last modified 2018-03-17

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