Targeted Photoswitchable Probe for Nanoscopy of Biological Structures
We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged microtubule structures with 25 nm resolution. The described probe in combination with the versatile SNAP-tag labeling opens new possibilities for imaging biological structures at the nanoscale.
Keywords: fluorescent probes ; microtubules ; protein structures ; SNAP-tag ; Storm ; Optical Reconstruction Microscopy ; Fusion Proteins ; Fluorescent-Probes ; Living Cells ; Storm ; Fluorophores ; Resolution ; Limit ; Tag
Dellagiacoma C., Lukinavicius G. and Bocchio N. have contributed equally to this work.
Record created on 2010-06-10, modified on 2016-08-08