Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged microtubule structures with ~25 nm resolution. The described probe in combination with the versatile SNAP-tag labeling opens new possibilities for imaging biological structures at the nanoscale.


Published in:
Chembiochem -Weinheim-, 11, 1361-1363
Year:
2010
Publisher:
Wiley-Blackwell
ISSN:
1439-4227
Keywords:
Note:
Dellagiacoma C., Lukinavicius G. and Bocchio N. have contributed equally to this work.
Laboratories:


Note: The status of this file is: Involved Laboratories Only


 Record created 2010-06-10, last modified 2018-09-13

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