Digital holography for second harmonic microscopy: application to 3D-tracking of nanoparticles

Retrieval of the amplitude and phase of electromagnetic waves made digital holographic microscopy (DHM) capable of revealing morphological details at ultrahigh resolution in the order of a few nanometers only and precisely measuring the refractive index across a sample (e.g. cell or neuron). In short,DHM added a new dimension to optical imaging,whic h explains why it is such an excellent instrument for metrological,but also for biological applications. We believe that DHM is,b y nature,ideally suited for nonlinear microscopy. In this work,w e review the advantages of DHM for nonlinear microscopy and present its application to determination of the axial position of nonlinear nanoparticles capable of second harmonic generation.


Published in:
Biophotonics: Photonic Solutions for Better Health Care II, 7715, 771505
Presented at:
Photonics Europe 2010, Bruxelles, Belgium, April 13-15, 2010
Year:
2010
Publisher:
SPIE
Keywords:
Laboratories:




 Record created 2010-04-21, last modified 2018-03-17

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