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research article
Surface-Plasmon-Mediated Single-Molecule Fluorescence Through a Thin Metallic Film
We demonstrate that fluorescence of single molecules in the nanometric vicinity of a thin gold film can be effectively excited and detected through the film with an epi-illumination scanning confocal micro- scope. A full theoretical treatment of the fluorescence signal indicates that both excitation and emission are surface-plasmon mediated. Remarkably, the number of photons detectable from chromophores perpendicular to the interface is enhanced by the presence of the metal.
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Type
research article
Authors
Stefani, F. D.
•
Vasilev, K.
•
Bocchio, N. L.
•
Stoyanova, N.
•
Kreiter, M.
Publication date
2005
Publisher
Published in
Volume
94
Article Number
023005
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
April 6, 2010