Surface-Plasmon-Mediated Single-Molecule Fluorescence Through a Thin Metallic Film
We demonstrate that fluorescence of single molecules in the nanometric vicinity of a thin gold film can be effectively excited and detected through the film with an epi-illumination scanning confocal micro- scope. A full theoretical treatment of the fluorescence signal indicates that both excitation and emission are surface-plasmon mediated. Remarkably, the number of photons detectable from chromophores perpendicular to the interface is enhanced by the presence of the metal.
Record created on 2010-04-06, modified on 2016-08-08