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  4. Evanescent optical fluorescence excitation: the role of photonic mode density
 
conference paper

Evanescent optical fluorescence excitation: the role of photonic mode density

Menges, Bernhard
•
Sato, Akihiro  
•
Knoll, Wolfgang
2007
Micro (MEMS) and Nanotechnologies for Defense and Security
Conference on Micro (MEMS) and Nanotechnologies for Defense and Security

In this paper we will discuss a few basic concepts concerning the use of evanescent optical fields for the excitation of fluorescent chromophores placed near the interface. The observation of enhanced fluorescence from chromophores excited by surface plasmon and waveguide modes will be presented and discussed. We attribute the enhancement to the near-field interaction between the chromophores and the increased photonic mode density by surface plasmon and waveguide modes. We determined limits of detection (LOD) of DNA hybridisation using the same sensor architecture by surface plasmon fluorescence spectroscopy (SPFS) and optical waveguide fluorescence spectroscopy (OWFS). Both SPFS and OWFS techniques have the same detection principle using an enhanced electromagnetic field to excite fluorophores and make it possible to monitor DNA hybridisation in real-time with high sensitivity. The relative photonic mode density of each mode was calculated under the resonance condition, and these values are reflected in the LOD values.

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Type
conference paper
DOI
10.1117/12.721103
Author(s)
Menges, Bernhard
Sato, Akihiro  
Knoll, Wolfgang
Date Issued

2007

Publisher

SPIE-INT SOC OPTICAL ENGINEERING

Published in
Micro (MEMS) and Nanotechnologies for Defense and Security
Series title/Series vol.

SPIE Proceedings; 6556

Start page

65560G

Subjects

evanescent optics

•

surface plasmon

•

waveguide modes

•

photonic mode density

•

fluorescence

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
LOB  
Event nameEvent placeEvent date
Conference on Micro (MEMS) and Nanotechnologies for Defense and Security

Orlando

April 10-12, 2007

Available on Infoscience
February 17, 2010
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/47537
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