Nuclear factor of activated T (NFAT) cells activity within CD4+ T cells is influenced by activation status and tissue localisation
Nuclear factor of activated T (NFAT) cells is a family of transcription factors important for the regulation of cytokine expression by CD4+ T cells. Whilst a number of studies have examined NFAT activity of in vitro generated CD4+ T helper (Th)1 and Th2 cells, regulation of NFAT during in vivo immune responses has yet to be elucidated. We show that NFAT activity in CD4+ T cells peaked at early time-points in the draining mediastinal lymph node of mice infected with influenza A (Flu) or Nippostrongylus brasiliensis (Nb). In contrast, low NFAT transcriptional activity was detected in CD4+ T cells isolated from the lung of either Flu or Nb infected mice, despite a greater proportion of cytokine-producing cells being present at this site. These findings indicate that the activation status and tissue microenvironment of effector CD4+ T cells can determine their requirement for NFAT-mediated transcription.
Keywords: Animals ; DNA/metabolism ; Influenza A virus/immunology/physiology ; Lung/cytology ; Lymph Nodes/cytology ; *Lymphocyte Activation ; Mice ; NFATC Transcription Factors/*metabolism ; Nippostrongylus ; Organ Specificity ; Orthomyxoviridae Infections/metabolism/virology ; Protein Binding ; Strongylida Infections/genetics/immunology/metabolism ; Th1 Cells/cytology/immunology/*metabolism ; Th2 Cells/cytology/immunology/*metabolism ; Transcription, Genetic
Harris, Nicola L Watt, Victoria MacLenachan, Sam Diehl, Sean Marsland, Benjamin J Rincon, Mercedes Le Gros, Graham Wellcome Trust/United Kingdom Research Support, Non-U.S. Gov't France Microbes and infection / Institut Pasteur Microbes Infect. 2006 Jan;8(1):232-7. Epub 2005 Sep 12.
Record created on 2010-01-06, modified on 2016-08-08