In this contribution we describe the application of Ink-Jet printing and Stencil Lithography in bionanotechnology. Both techniques are alternative patterning methods that can be used for the fabrication of biocompatible micro- and nanostructures out of the costly and restricted clean room environment. The applications presented in this contribution are 1) the cell patterning using Au dot arrays deposited on PDMS,by stencil lithography, 2) the fabrication of biosensors based on localized surface plasmon resonance in Au nanodots deposited by stencil lithography and 3) the printing of cells and biomolecules by InkJet printing.