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research article

Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram

Colomb, Tristan  
•
Kühn, Jonas
•
Charrière, Florian  
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2006
Optics Express

In this paper we present a new method to achieve quantitative phase contrast imaging in Digital Holographic Microscopy (DHM) that allows to compensate for phase aberrations and image distortion by recording of a single reference hologram.We demonstrate that in particular cases in which the studied specimen does not have abrupt edges, the specimen’s hologram itself can be used as reference hologram. We show that image distortion and phase aberrations introduced by a lens ball used as microscope objective are completely suppressed with our method. Finally the concept of self-conjugated reference hologram is applied on a biological sample (Trypanosoma Brucei) to maintain a spatial phase noise level under 3 degrees.

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Type
research article
DOI
10.1364/OE.14.004300
Web of Science ID

WOS:000237608600011

Author(s)
Colomb, Tristan  
Kühn, Jonas
Charrière, Florian  
Depeursinge, Christian  
Marquet, Pierre
Aspert, Nicolas
Date Issued

2006

Published in
Optics Express
Volume

14

Issue

10

Start page

4300

End page

4306

Subjects

[MVD]

•

Aberration compensation

•

Computer holography

•

Interference microscopy

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LOA  
Available on Infoscience
July 20, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/41603
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