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conference paper

Use of digital holographic microscopy in tomography

Charrière, F.  
•
Montfort, F.  
•
Kühn, J.
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2006
Biophotonics and New Therapy Frontiers
Photonics Europe

Digital Holographic Microscopy (DHM) provides three-dimensional (3D) images with a high vertical accuracy in the nanometer range and a diffracted limited transverse resolution. This paper focuses on 3 different tomographic applications based on DHM. First, we show that DHM can be combined with time gating: a series of holograms is acquired at different depths by varying the reference path length, providing after reconstruction images of slices at different depths in the specimen thanks to the short coherence length of the light source. Studies on enucleated porcine eyes will be presented. Secondly, we present a tomography based on the addition of several reconstructed wavefronts measured with DHM at different wavelengths. Each wavefront phase is individually adjusted to be equal in a given plane of interest, resulting in a constructive addition of complex waves in the selected plane and destructive addition in the others. Varying the plane of interest enables the scan of the object in depth. Thirdly, DHM is applied to perform optical diffraction tomography of a pollen grain: transmission phase images are acquired for different orientations of the rotating sample, then the 3D refractive index spatial distribution is computed by inverse radon transform. The presented works will exemplify the versatility of DHM, but above all its capability of providing quantitative tomographic data of biological specimen in a quick, reliable and non-invasive way.

  • Details
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Type
conference paper
DOI
10.1117/12.662011
Web of Science ID

WOS:000238042700026

Author(s)
Charrière, F.  
Montfort, F.  
Kühn, J.
Colomb, T.  
Marian, A.
Cuche, E.  
Marquet, P.
Depeursinge, C.  
Date Issued

2006

Publisher

SPIE

Published in
Biophotonics and New Therapy Frontiers
ISBN of the book

0-8194-6247-0

Volume

6191

Start page

O1910

End page

O1910

Subjects

[MVD]

•

digital holography

•

tomography

•

3D imaging

•

cell imaging

•

OPTICAL COHERENCE TOMOGRAPHY

•

CONTRAST

•

RECONSTRUCTION

URL

URL

http://spie.org/x648.html?product_id=662011
Editorial or Peer reviewed

NON-REVIEWED

Written at

EPFL

EPFL units
LOA  
Event nameEvent placeEvent date
Photonics Europe

Strasbourg

April 3-7, 2006

Available on Infoscience
July 20, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/41596
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