Point spread function model for microscopic image deconvolution in digital holographic microscopy

A simple model is proposed in order to evaluate the complex amplitude point spread function (intensity and phase) of a microscope objective. The model is based on the Fresnel diffraction theory and takes also into account the possible optical aberrations. Experimental evaluation of this amplitude point spread function has been carried out by using a holographic set-up and 66 nanometers gold spheres as punctual objects. The measured values are then compared with the theoretical predicted model.


Published in:
Novel Optical Instrumentation for Biomedical Applications, 5143, 202-209
Presented at:
European Conferences on Biomedical Optics, Munich
Year:
2003
Publisher:
SPIE
ISBN:
0277-786X 0-8194-5013-8
Keywords:
Laboratories:




 Record created 2009-07-20, last modified 2018-01-28

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