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  4. High Resolution Mass Spectrometry for Studying the Interactions of Cisplatin with Oligonucleotides
 
research article

High Resolution Mass Spectrometry for Studying the Interactions of Cisplatin with Oligonucleotides

Egger, Alexander E.
•
Hartinger, Christian G.
•
Ben Hamidane, Hisham  
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2008
Inorganic Chemistry

Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) has been used to probe the interaction of the anticancer drug cisplatin with oligonucleotides. The binding kinetics, the nature of the adducts formed, and the location of the binding site within the specifically designed double-stranded DNA oligonucleotides, ds(GTATTGGCACGTA) and ds(GTACCGGTGTGTA), were detd. by recording mass spectra over time and/or employing tandem mass spectrometry (MS/MS). The FT-ICR MS studies show that binding to DNA takes place via a [Pt(NH3)2Cl]+ intermediate prior to formation of bifunctional [Pt(NH3)2]2+ adducts. Tandem MS reveals that the major binding sites correspond to GG and GTG, the known preferred binding sites for cisplatin, and demonstrates the preference for binding to guanosine within the oligonucleotide. The obtained results are discussed and compared to published data obtained by other mass spectrometric techniques, NMR spectroscopy and x-ray crystallog.

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Type
research article
DOI
10.1021/ic801371r
Web of Science ID

WOS:000260791100058

Author(s)
Egger, Alexander E.
Hartinger, Christian G.
Ben Hamidane, Hisham  
Tsybin, Yury O.  
Keppler, Bernhard K.
Dyson, Paul J.  
Date Issued

2008

Published in
Inorganic Chemistry
Volume

47

Issue

22

Start page

10626

End page

10633

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LSMB  
LCOM  
Available on Infoscience
July 15, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/41370
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