000139485 001__ 139485
000139485 005__ 20180913055336.0
000139485 02470 $$2ISI$$a000271922700006
000139485 0247_ $$2doi$$a10.1016/j.vaccine.2009.06.019
000139485 037__ $$aARTICLE
000139485 245__ $$aRespiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells
000139485 269__ $$a2009
000139485 260__ $$c2009
000139485 336__ $$aJournal Articles
000139485 520__ $$aAlthough respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV fusion protein (rRSV-F) are described. The recombinant protein was produced by transient gene expression (TGE) in suspension-adapted human embryonic kidney cells (HEK-293E) in 4L orbitally shaken bioreactors. It was then purified and formulated in immunostimulating reconstituted influenza virosomes (IRIVs). The candidate vaccine induced anti-RSV-F neutralizing antibodies in mice, and challenge studies in cotton rats are ongoing. If successful in preclinical and clinical trials, this will be the first recombinant subunit vaccine produced by large-scale TGE in mammalian cells.
000139485 6531_ $$aTransient transfection
000139485 6531_ $$aRecombinant protein
000139485 6531_ $$aRSV vaccine
000139485 6531_ $$aVirosomes
000139485 6531_ $$aLarge-Scale Production
000139485 6531_ $$aInfluenza Virosomes
000139485 6531_ $$aDelivery-System
000139485 6531_ $$aEfficient
000139485 6531_ $$aImmunity
000139485 6531_ $$aPurification
000139485 6531_ $$aTechnology
000139485 6531_ $$aInfection
000139485 6531_ $$aChildren
000139485 700__ $$aNallet, S
000139485 700__ $$aAmacker, M
000139485 700__ $$aWesterfeld, N
000139485 700__ $$0240397$$aBaldi, L$$g117359
000139485 700__ $$aKönig, I
000139485 700__ $$0240882$$aHacker, DL$$g152132
000139485 700__ $$aZaborosch, C
000139485 700__ $$aZurbriggen, R
000139485 700__ $$0240398$$aWurm, FM$$g107554
000139485 773__ $$j27$$k46$$q6415–6419$$tVaccine
000139485 909C0 $$0252009$$pLBTC$$xU10180
000139485 909CO $$ooai:infoscience.tind.io:139485$$particle
000139485 917Z8 $$x148230
000139485 937__ $$aLBTC-ARTICLE-2009-002
000139485 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000139485 980__ $$aARTICLE