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research article
Redox-cycling type electrochemiluminescence in aqueous medium. A new principle for the detection of proteins labeled with a ruthenium chelate
The first observation of electrochemiluminescence (ecl) based on the redox-cycling of a ruthenium chelate covalently linked to a protein and placed in aqueous medium containing neither solvent nor reducing compound is reported here. Ecl experiments based on redox-cycling were performed in phosphate buffer at carbon interdigitated microelectrode arrays. The light intensity represented 31% of that obtained using a non complexed Ru(bpy)3 2+ solution prepared in phosphate buffer containing proteins.
Type
research article
Authors
Michel, P.
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Koudelka-Hep, M.
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Faehnrich, K. A.
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O'Sullivan, C. K.
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Guilbault, G. G.
Publication date
1999
Published in
Volume
474
Issue
2
Start page
192
End page
194
Note
201
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
May 12, 2009
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