The invention relates to methods that enable identification of modulators of nuclear receptor (NR) activity and to methods for treating and/or preventing diseases or pathologic conditions associated with cell types that express said nuclear receptors. More particularly, the invention concerns to methods enabling the identification of compounds that induce or inhibit the recruitment of the p160 family cofactors, especially TIF-2 and/or SRCs cofactors and that are useful for modulating the PPAR-gamma biological activity and for treating and/or preventing various PPAR-gamma related diseases and conditions, including metabolic or cell proliferative disorders