In this unit, a procedure for post-mortem examination of mice and tissue collection is provided. This procedure is performed for post-mortem analysis of anatomical defects (necropsy) and histological analysis and/or tissue collection destined for molecular biology applications. In both cases, tissue preservation is the major issue, but the way to achieve it depends on the objective. When histological analysis is the aim, tissue preservation is achieved by rapid transfer into fixative solutions. In contrast, molecular biology applications require rapid freezing of tissue samples to preserve mRNA integrity. Consequently, performing both procedures simultaneously may be at the expense of the final product quality
Type
book part or chapter
Authors
Editors
Ausubel, F.M.
•
Struhl, K.
•
Smith, J.A
•
Seidman, J.G.
•
Moore, D.D.
•
Kingston, R.E.
Publication date
2007
Publisher
Published in
Current Protocols in Molecular Biology
Start page
29A.4
Series title/Series vol.
Current Protocols
EPFL units
Available on Infoscience
April 20, 2009
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