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research article
Photobleaching-free infrared near-field microscopy localizes molecules in neurons
High-resolution detection of specific molecules in cells is a major challenge in biology. We show that infrared scanning near-field microscopy can detect the spatial distribution of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid–type glutamate receptor clusters on hippocampal neurons. The GluR2 subunits were labeled with the die Alexa 488 and high-resolution infrared micrographs were taken at 6.25 µm. The absence of photobleaching makes this approach suitable for a long-term observation and allows to localize different infrared-absorbing molecules over the complex background of other cell components.
Type
research article
Web of Science ID
WOS:000262605800166
Authors
Generosi, Johanna
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Kropf, Michel
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Hirling, Harald
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Catsicas, Stefan
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Tolk, Norman H.
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Piston, David W.
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Cricenti, Antonio
Publication date
2008
Published in
Volume
104
Article Number
106102
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
March 12, 2009
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