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research article
In vitro binding of nucleolin to double-stranded telomeric DNA
We have purified a 100 kDa protein, resolved in a Southwestern binding screen of total nuclear proteins from Hela cells with double-stranded human telomeric probe. A polyclonal antiserum raised by this protein recognizes purified nucleolin and stains nucleoli in growing Hela cells. We demonstrate that a truncated form of human nucleolin and a purified deletion derivative of mouse nucleolin bind in vitro to duplex telomeric DNA. This study suggests a new link between telomeres and the nucleolus.
Type
research article
Authors
Publication date
2000
Volume
268
Issue
3
Start page
909
End page
15
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
February 19, 2009
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