In vitro binding of nucleolin to double-stranded telomeric DNA

We have purified a 100 kDa protein, resolved in a Southwestern binding screen of total nuclear proteins from Hela cells with double-stranded human telomeric probe. A polyclonal antiserum raised by this protein recognizes purified nucleolin and stains nucleoli in growing Hela cells. We demonstrate that a truncated form of human nucleolin and a purified deletion derivative of mouse nucleolin bind in vitro to duplex telomeric DNA. This study suggests a new link between telomeres and the nucleolus.


Published in:
Biochemical and biophysical research communications, 268, 3, 909-15
Year:
2000
ISSN:
0006-291X
Laboratories:




 Record created 2009-02-19, last modified 2018-09-13


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)