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  4. Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD
 
research article

Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD

Steiner, Pascal  
•
Sarria, J-C Floyd  
•
Glauser, Liliane  
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2002
The Journal of cell biology

Although correct cycling of neuronal membrane proteins is essential for neurite outgrowth and synaptic plasticity, neuron-specific proteins of the implicated endosomes have not been characterized. Here we show that a previously cloned, developmentally regulated, neuronal protein of unknown function binds to syntaxin 13. We propose to name this protein neuron-enriched endosomal protein of 21 kD (NEEP21), because it is colocalized with transferrin receptors, internalized transferrin (Tf), and Rab4. In PC12 cells, NEEP21 overexpression accelerates Tf internalization and recycling, whereas its down-regulation strongly delays Tf recycling. In primary neurons, NEEP21 is localized to the somatodendritic compartment, and, upon N-methyl-d-aspartate (NMDA) stimulation, the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor subunit GluR2 is internalized into NEEP21-positive endosomes. NEEP21 down-regulation retards recycling of GluR1 to the cell surface after NMDA stimulation of hippocampal neurons. In summary, NEEP21 is a neuronal protein that is localized to the early endosomal pathway and is necessary for correct receptor recycling in neurons.

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Type
research article
DOI
10.1083/jcb.200202022
Web of Science ID

WOS:000176528400009

PubMed ID

12070131

Author(s)
Steiner, Pascal  
Sarria, J-C Floyd  
Glauser, Liliane  
Magnin, Sarah  
Catsicas, Stefan  
Hirling, Harald  
Date Issued

2002

Published in
The Journal of cell biology
Volume

157

Issue

7

Start page

1197

End page

209

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
PTBIOP  
Available on Infoscience
February 19, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/35530
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