High volume confinement in two-photon total-internal- reflection fluorescence correlation spectroscopy

We report results on two-photon total-internal-reflection fluorescence correlation spectroscopy with radially polarized light. The combination of liquid crystal spatial light modulator, providing radial polarization, with ultrafast laser (picosecond Nd:GdVO4 laser) allowed us to take the advantage of nonlinear optical contrast mechanisms to suppress the side-lobe energy specific for radial polarization and reduce the effective excited volume twice compared to one-photon evanescent wave excitation in fluorescence correlation spectroscopy.


Published in:
Applied Physics Letters, 94, 8, 083902
Year:
2009
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 Record created 2009-01-09, last modified 2018-03-17

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