Calmodulin-tagged phage and two-filter sandwich assays for the identification of enzymatic activities
2003
Abstract
The procedures for calmodulin-tagged phage and two-filter sandwich assays for the isolation of enzymic activities are presented. In the first method, enzymes are displayed on filamentous phage, and the reaction substrate is anchored on the calmodulin-tagged phage-enzymes using a calmodulin binding peptide. In the second method, enzyme mutants are expressed in bacterial colonies on a porous "master" filter, and the enzymes are released and diffuse to a second reaction filter that closely contacts the master filter. [on SciFinder (R)]
Details
Title
Calmodulin-tagged phage and two-filter sandwich assays for the identification of enzymatic activities
Author(s)
Heinis, Christian ; Bertschinger, Julian ; Neri, Dario
Published in
Directed Enzyme Evolution
Series
Methods in Molecular Biology
Pages
313-328
Date
2003
Publisher
Totowa, Humana Press
ISBN
1-58829-286-X
Keywords
Bacteriophage; Filamentous bacteriophage (calmodulin-tagged phage and two-filter sandwich assays for identification of enzyme activities); Enzymes Role: ANT (Analyte); BSU (Biological study unclassified); ANST (Analytical study); BIOL (Biological study) (calmodulin-tagged phage and two-filter sandwich assays for identification of enzyme activities); Calmodulins Role: ARU (Analytical role unclassified); BUU (Biological use unclassified); ANST (Analytical study); BIOL (Biological study); USES (Uses) (calmodulin-tagged phage and two-filter sandwich assays for identification of enzyme activities); enzyme activity calmodulin tagged phage two filter sandwich assay
Note
CAN 140:212803 7-1 Enzymes Institute of Pharmaceutical Sciences,Swiss Federal Institute of Technology,Zurich,Switz. Journal 1064-3745 written in English. 37340-95-7 (Biotin ligase) Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (calmodulin-tagged phage and two-filter sandwich assays for identification of enzyme activities)
Laboratories
LPPT
Record Appears in
Scientific production and competences > SB - School of Basic Sciences > ISIC - Institute of Chemical Sciences and Engineering > LPPT - Laboratory of Therapeutic Proteins and Peptides
Work outside EPFL
Book chapters
Published
Work outside EPFL
Book chapters
Published
Record creation date
2008-11-17