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  4. A DMSO-sensitive conditional mutant of the fission yeast orthologue of the Saccharomyces cerevisiae SEC13 gene is defective in septation
 
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research article

A DMSO-sensitive conditional mutant of the fission yeast orthologue of the Saccharomyces cerevisiae SEC13 gene is defective in septation

Poloni, D.
•
Simanis, V.  
2002
FEBS Letters

Dissection of complex processes using model organisms such as yeasts relies heavily upon the use of conditional mutants. We have generated a collection of fission yeast mutants sensitive to dimethylsulphoxide (DMSO). Among these we have found a mutant in the Schizosaccharomyces pombe orthologue of the Saccharomyces cerevisiae SEC13 gene, which fails to cleave the division septum. Generation of a null allele demonstrates that the S. pombe sec13 gene is essential.

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Type
research article
DOI
10.1016/S0014-5793(01)03285-9
Author(s)
Poloni, D.
•
Simanis, V.  
Date Issued

2002

Published in
FEBS Letters
Volume

511

Issue

1-3

Start page

85

End page

9

Subjects

Amino Acid Sequence

•

Cell Division/drug effects/genetics

•

Cell Survival/genetics

•

Cloning

•

Molecular

•

Dimethyl Sulfoxide/*pharmacology

•

Genes

•

Essential/genetics

•

Membrane Proteins/chemistry/genetics/*metabolism

•

Molecular Sequence Data

•

Mutagenesis/genetics

•

Mutation/drug effects/*genetics

•

Nuclear Pore Complex Proteins

•

Saccharomyces cerevisiae/*genetics

•

Saccharomyces cerevisiae Proteins

•

Schizosaccharomyces/*cytology/drug effects/*genetics/metabolism

•

Schizosaccharomyces pombe Proteins/chemistry/genetics/metabolism

•

Time Factors

Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
UPSIM  
Available on Infoscience
October 21, 2008
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/30360
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