A DMSO-sensitive conditional mutant of the fission yeast orthologue of the Saccharomyces cerevisiae SEC13 gene is defective in septation
2002
Résumé
Dissection of complex processes using model organisms such as yeasts relies heavily upon the use of conditional mutants. We have generated a collection of fission yeast mutants sensitive to dimethylsulphoxide (DMSO). Among these we have found a mutant in the Schizosaccharomyces pombe orthologue of the Saccharomyces cerevisiae SEC13 gene, which fails to cleave the division septum. Generation of a null allele demonstrates that the S. pombe sec13 gene is essential.
Détails
Titre
A DMSO-sensitive conditional mutant of the fission yeast orthologue of the Saccharomyces cerevisiae SEC13 gene is defective in septation
Auteur(s)
Poloni, D. ; Simanis, V.
Publié dans
FEBS Letters
Volume
511
Numéro
1-3
Pages
85-9
Date
2002
Mots-clés (libres)
Amino Acid Sequence; Cell Division/drug effects/genetics; Cell Survival/genetics; Cloning; Molecular; Dimethyl Sulfoxide/*pharmacology; Genes; Essential/genetics; Membrane Proteins/chemistry/genetics/*metabolism; Molecular Sequence Data; Mutagenesis/genetics; Mutation/drug effects/*genetics; Nuclear Pore Complex Proteins; Saccharomyces cerevisiae/*genetics; Saccharomyces cerevisiae Proteins; Schizosaccharomyces/*cytology/drug effects/*genetics/metabolism; Schizosaccharomyces pombe Proteins/chemistry/genetics/metabolism; Time Factors
Laboratoires
UPSIM
Le document apparaît dans
Production scientifique et compétences > SV - Faculté des sciences de la vie > ISREC - Institut suisse de recherche expérimentale sur le cancer > UPSIM - Unité du Prof. Simanis
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Articles de journaux
Publié
Publications validées par des pairs
Travail produit à l'EPFL
Articles de journaux
Publié
Date de création de la notice
2008-10-21