Extended focus Fourier domain optical coherence microscopy assists developmental biology

We present a novel detection scheme for Fourier domain optical coherence microscopy (FDOCM). A Bessel-like interference pattern with a strong central lobe was created with an axicon lens. This pattern was then imaged by a telescopic system into the sample space to obtain a laterally highly confined illumination needle, extending over a long axial range. For increased efficiency, the detection occurs decoupled from the illumination, avoiding a double pass through the axicon. Nearly constant transverse resolution of ~1.5µm along a focal range of 200µm with a maximum sensitivity of 105dB was obtained. A broad bandwidth Ti:Sapphire laser allowed for an axial resolution of 3µm in air, providing the nearly isotropic resolution necessary to access the microstructure of biological tissues. Together with the speed- and sensitivity-advantage of FDOCT, this system can perform in vivo measurements in a minimally invasive way. Tomograms of the mouse mammary gland and the mouse follicle, recorded in vitro, revealed biologically relevant structural details. Images acquired with classical microscopy techniques, involving stained and fluorescent samples, validate these structures and emphasize the high contrast of the tomograms. It is comparable to the contrast achieved with classical techniques, but employing neither staining, labeling nor slicing of the samples, stressing the high potential of FDOCM for minimally invasive in vivo small animal imaging.


Published in:
Proceedings of SPIE: Advances in OCT System Technology II, 6627, 66271H
Presented at:
Optical Coherence Tomography and Coherence Techniques III, Munich, Germany, Sunday 17 June 2007
Year:
2007
Publisher:
SPIE
Laboratories:


Note: The status of this file is: Involved Laboratories Only


 Record created 2008-07-03, last modified 2018-09-13

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