Loading...
research article
AMPA receptor imaging by infrared scanning near-field optical microscopy
Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 µm wavelength, corresponding to the maker's strong infrared absorption. The use of infrared light avoids photobleaching and makes this approach suitable for long-term observations; this technique can be used in general to localize different IR-absorbing molecules over the complex background of other cell components.
Type
research article
Web of Science ID
WOS:000257704500021
Authors
Generosi, Johanna
•
Kropf, Michel
•
•
Hirling, Harald
•
Catsicas, Stefan
•
•
Tolk, Norman H.
•
Piston, David W.
•
Cricenti Phys., Antonio
Publication date
2008
Published in
Volume
5
Issue
8
Start page
2641
End page
2644
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
June 10, 2008
Use this identifier to reference this record