Preparation of nucleic acid templates for solid phase amplification

The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support


Year:
2010
Note:
Also published as: WO2007060456 / EP1957668
Other identifiers:
EPO Family ID: 35601246
Laboratories:




 Record created 2008-05-13, last modified 2018-09-13


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