000117703 001__ 117703
000117703 005__ 20181203021112.0
000117703 0247_ $$2doi$$a10.1038/82593
000117703 037__ $$aARTICLE
000117703 245__ $$aTargeted inversion of a polar silencer within the HoxD complex re-allocates domains of enhancer sharing
000117703 269__ $$a2000
000117703 260__ $$c2000
000117703 336__ $$aJournal Articles
000117703 500__ $$aDepartment of Zoology and Animal Biology, University of Geneva, Sciences III, Geneva, Switzerland.
000117703 520__ $$aMammalian Hox genes are clustered at four genomic loci. During development, neighbouring genes are coordinately regulated by global enhancer sequences, which control multiple genes at once, as exemplified by the expression of series of contiguous Hoxd genes in either limbs or gut. The link between vertebrate Hox gene transcription and their clustered distribution is poorly understood. Experimental and comparative approaches have revealed that various mechanisms, such as gene clustering or global enhancer sequences, might have constrained this genomic organization and stabilized it throughout evolution. To understand what restricts the effect of a particular enhancer to a precise set of genes, we generated a loxP/Cre-mediated targeted inversion within the HoxD cluster. Mice carrying the inversion showed a reciprocal re-assignment of the limb versus gut regulatory specificities, suggesting the presence of a silencer element with a unidirectional property. This polar silencer appears to limit the number of genes that respond to one type of regulation and thus indicates how separate regulatory domains may be implemented within intricate gene clusters.
000117703 700__ $$aKmita, M.
000117703 700__ $$aKondo, T.
000117703 700__ $$g141236$$aDuboule, D.$$0240276
000117703 773__ $$j26$$tNat Genet$$k4$$q451-4
000117703 909C0 $$xU11705$$0252099$$pUPDUB
000117703 909CO $$pSV$$particle$$ooai:infoscience.tind.io:117703
000117703 937__ $$aUPDUB-ARTICLE-2000-002
000117703 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000117703 980__ $$aARTICLE