Field confinement with aberration correction for solid immersion lens based fluorescence correlation spectroscopy

The solid immersion lens (SIL) as a tool for increasing the field confinement as well as providing optimal performance by aberration compensation in a confocal fluorescence correlation spectroscopy (FCS) system is illustrated here. Using Zernike polynomials we show that aberration compensation and the resultant pre-shaping of the incident wavefront enables near diffraction-limited performance. This is explained based on vectorial computations for high apertures in the Debye approximation. The obtained axial resolution parameters are compared with the obtained diffusion times in a SIL-FCS experiment for measurements in solutions done at the single molecule level.


Published in:
Optics Communications, 271, 2, 462-469
Year:
2007
Keywords:
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 Record created 2008-02-25, last modified 2018-07-07


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