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research article
Full on-chip nanoliter immuno-assay by geometrical magnetic trapping of nanoparticle chains
2008
We propose an original concept to perform a complete on-chip sandwich immunoassay on magnetic nanoparticles that are self-assembled in chains in a uniform magnetic field. The magnetic chains are retained over periodically enlarged cross sections of a microfluidic channel. Thereby they strongly interact with the flow and rapidly capture the total of a low number of target molecules from nanoliter sample volumes. As an example, we demonstrate the detection of murine monoclonal antibodies in a noncompetitive sandwich immunoassay with a detection limit of 1 ng mL-1 in nanoliters of hybridoma cell culture medium.
Type
research article
Web of Science ID
WOS:000254969100034
Authors
Publication date
2008
Published in
Volume
80
Issue
8
Start page
2905
End page
2910
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
January 16, 2008
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