Full on-chip nanoliter immuno-assay by geometrical magnetic trapping of nanoparticle chains

We propose an original concept to perform a complete on-chip sandwich immunoassay on magnetic nanoparticles that are self-assembled in chains in a uniform magnetic field. The magnetic chains are retained over periodically enlarged cross sections of a microfluidic channel. Thereby they strongly interact with the flow and rapidly capture the total of a low number of target molecules from nanoliter sample volumes. As an example, we demonstrate the detection of murine monoclonal antibodies in a noncompetitive sandwich immunoassay with a detection limit of 1 ng mL-1 in nanoliters of hybridoma cell culture medium.

Published in:
Anal. Chem., 80, 8, 2905–2910
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 Record created 2008-01-16, last modified 2018-03-17

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