Inducible gene targeting in mice

A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.


Published in:
Science, 269, 5229, 1427-9
Year:
1995
Note:
Institute for Genetics, University of Cologne, Germany.
Laboratories:




 Record created 2007-12-12, last modified 2018-12-03


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