A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.
Type
research article
Authors
Publication date
1995
Published in
Volume
269
Issue
5229
Start page
1427
End page
1429
Note
Institute for Genetics, University of Cologne, Germany.
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
December 12, 2007
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