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research article
Large-scale transient expression in mammalian cells for recombinant protein production
Large-scale transient expression from mammalian cells is a new technology. Breakthroughs have been achieved for non-viral delivery methods: transfections can now be done at the 1-10 L scale with mammalian cells grown in suspension. Production of 1-20 mg/L of recombinant protein have been obtained in stirred bioreactors. Modified alphaviruses have provided a fast and efficient expression technology based on viral vectors.
Type
research article
Web of Science ID
WOS:000079523500009
PubMed ID
10209142
Authors
Publication date
1999
Published in
Volume
10
Issue
2
Start page
156
End page
9
Note
Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland.
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
June 5, 2007
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