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  4. Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
 
research article

Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms

Cuche, E.  
•
Marquet, P.
•
Depeursinge, C.  
1999
Applied Optics

We present a digital method for holographic microscopy involving a CCD camera as a recording device. Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation. For phase-contrast imaging the reconstruction method involves the computation of a digital replica of the reference wave. A digital method for the correction of the phase aberrations is presented. We present a detailed description of the reconstruction procedure and show that the transverse resolution is equal to the diffraction limit of the imaging system.

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Type
research article
DOI
10.1364/AO.38.006994
Web of Science ID

WOS:000084023500003

Author(s)
Cuche, E.  
Marquet, P.
Depeursinge, C.  
Date Issued

1999

Published in
Applied Optics
Volume

38

Issue

34

Start page

6994

End page

7001

Subjects

[MVD]

•

Detectors : CCD, charge-coupled device

•

Holography : Holography

•

Holography : Aberration compensation

•

Imaging systems : Microscopy

•

Other areas of optics : Phase

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LOA  
Available on Infoscience
July 20, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/41493
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