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research article

Extended depth-of-focus by digital holographic microscopy

Colomb, Tristan  
•
Pavillon, Nicolas  
•
Kühn, Jonas
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2010
Optics Letters

A recurrent problem in microscopy is the finite depth-of-focus linked to the NA of microscope objectives. Digital holographic microscopy (DHM) has the unique feature of being able to numerically change the focus from a single hologram without the need of moving the sample. Extended depth of focus of amplitude images has been demonstrated, but it has marginal interest for the metrological application of DHM that needs the topography. In this Letter, we demonstrate that DHM is able to provide not only extended depth-of-focus amplitude images but extended focused complex data from which the topography is computed. For this purpose, reflection and transmission measurements on micro-optics (microlens and retroreflector) performed by using standard reconstruction or the extended focused complex data are compared. These experiments demonstrate that DHM measures, from a single hologram acquisition, the accurate sample topography on a numerically increased depth-of-focus.

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Type
research article
DOI
10.1364/OL.35.001840
Web of Science ID

WOS:000278230400038

Author(s)
Colomb, Tristan  
Pavillon, Nicolas  
Kühn, Jonas
Cuche, Etienne  
Depeursinge, Christian  
Emery, Yves
Date Issued

2010

Publisher

Optical Society of America

Published in
Optics Letters
Volume

35

Issue

11

Start page

1840

End page

1842

Subjects

[MVD]

•

Image processing : Image enhancement

•

Microscopy : Interference microscopy

•

Holography : Digital holography

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LOA  
Available on Infoscience
April 29, 2010
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/49816
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