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  4. Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy
 
research article

Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Marquet, Pierre
•
Rappaz, Benjamin  
•
Magistretti, Pierre J.
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2005
Optics Letters

We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsewhere [Cuche et al.,Appl.Opt.38, 6994 (1999)]. DHM images of living cells in culture are shown for what is to our knowledge the first time. They represent the distribution of the optical path length over the cell, which has been measured with subwavelength accuracy. These DHM images are compared with those obtained by use of the widely used phase contrast and Nomarski differential interference contrast techniques.

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Type
research article
DOI
10.1364/OL.30.000468
Web of Science ID

WOS:000227371800006

Author(s)
Marquet, Pierre
Rappaz, Benjamin  
Magistretti, Pierre J.
Cuche, Etienne  
Emery, Yves
Colomb, Tristan  
Depeursinge, Christian  
Date Issued

2005

Published in
Optics Letters
Volume

30

Issue

5

Start page

468

End page

470

Subjects

[MVD]

•

Holography : Holography

•

Holography : Aberration compensation

•

Image processing : Digital image processing

•

Imaging systems : Coherence imaging

•

Medical optics and biotechnology : Coherence imaging

Editorial or Peer reviewed

REVIEWED

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EPFL

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July 20, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/41571
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