The role of plants in sequestering carbon is a critical component in mitigating climate change. A key aspect of this role involves plant nitrogen (N) uptake (Nup) and N use efficiency (NUE), as these factors directly influence the capacity of plants to store carbon. However, the additive contribution of N deposition, soil factors (biotic and abiotic), and climate to the plant N cycle remains inadequately understood, introducing significant uncertainties into climate change projections. Here, we used ground-based observations across 159 field experiments (including above and belowground information) to calculate Nup and NUE and identify their main drivers in natural ecosystems. We found that global plant Nup is primarily driven by N deposition, mean temperature, and precipitation, with Nup increasing in warmer and wetter areas. In contrast, NUE is driven by soil biotic and abiotic factors. Specifically, NUE decreased with the intensity of colonization by arbuscular mycorrhizal fungi and increased with soil pH and soil microbial stocks. Nup and NUE presented opposite latitudinal distributions, with Nup higher on tropical latitudes and NUE higher towards the poles. Total soil N stocks were not found to be a driver of Nup or NUE. We also compared our results with TRENDY models and found that models may overestimate Nup by similar to 100 TgNyr-1 in the tropics and triple the standard deviation at boreal latitudes. Our findings emphasize the effect of N deposition and soil microbes that, in addition to climate and soil pH, are crucial for accurately predicting ecosystems' capacity to sequester carbon and mitigate climate change at a global scale.