Repository logo

Infoscience

  • English
  • French
Log In
Logo EPFL, École polytechnique fédérale de Lausanne

Infoscience

  • English
  • French
Log In
  1. Home
  2. Academic and Research Output
  3. Patents
  4. Cumulant microscopy
 
patent

Cumulant microscopy

Geissbuehler, Stefan
•
Dellagiacoma, Claudio  
•
Geissbuehler, Matthias
Show more
2012

The invention describes a method and a microscopy system for imaging and analysing stochastically and independently blinking point-like emitters. A multiple-order cumulants analysis in conjunction with an established blinking model enables the extraction of super-resolved environment-related parameter maps, such as molecular state lifetimes, concentration and brightness distributions of the emitter. In addition, such parameter maps can be used to compensate for the non-linear brightness and blinking response of higher-order cumulant images - used for example in Super-resolution Optical Fluctuation Imaging (SOFI) - to generate a balanced image contrast. Structures that otherwise would be masked by brighter regions in the conventional cumulant image become visible in the balanced cumulant image. The invention furthermore provides a method for the spectral unmixing of multi- colour samples using spectral cross-cumulants.

  • Details
  • Metrics
Type
patent
EPO Family ID

46208106

Author(s)
Geissbuehler, Stefan
Dellagiacoma, Claudio  
Geissbuehler, Matthias
Lasser, Theo  
Note

Alternative title(s) : (de) Kumulantenmikroskopie (fr) Microscopie des cumulants

TTO classification

TTO:6.1091

EPFL units
LOB  
AVP-R-TTO  
DOICountry codeKind codeDate issued

EP2721441

EP

B1

2019-09-18

US9658442

US

B2

2017-05-23

US2014198198

US

A1

2014-07-17

EP2721441

EP

A1

2014-04-23

WO2012172440

WO

A1

2012-12-20

EP2535755

EP

A1

2012-12-19

Available on Infoscience
September 22, 2015
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/118325
Logo EPFL, École polytechnique fédérale de Lausanne
  • Contact
  • infoscience@epfl.ch

  • Follow us on Facebook
  • Follow us on Instagram
  • Follow us on LinkedIn
  • Follow us on X
  • Follow us on Youtube
AccessibilityLegal noticePrivacy policyCookie settingsEnd User AgreementGet helpFeedback

Infoscience is a service managed and provided by the Library and IT Services of EPFL. © EPFL, tous droits réservés