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research article

A microfluidic flow-cell for the study of the ultrafast dynamics of biological systems

Chauvet, Adrien  
•
Tibiletti, Tania
•
Caffarri, Stefano
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2014
Review Of Scientific Instruments

The study of biochemical dynamics by ultrafast spectroscopic methods is often restricted by the limited amount of liquid sample available, while the high repetition rate of light sources can induce photodamage. In order to overcome these limitations, we designed a high flux, sub-ml, capillary flow-cell. While the 0.1 mm thin window of the 0.5 mm cross-section capillary ensures an optimal temporal resolution and a steady beam deviation, the cell-pump generates flows up to similar to 0.35 ml/s that are suitable to pump laser repetition rates up to similar to 14 kHz, assuming a focal spot-diameter of 100 mu m. In addition, a decantation chamber efficiently removes bubbles and allows, via septum, for the addition of chemicals while preserving the closed atmosphere. The minimal useable amount of sample is similar to 250 mu l. (C) 2014 AIP Publishing LLC.

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Type
research article
DOI
10.1063/1.4899120
Web of Science ID

WOS:000344594200018

Author(s)
Chauvet, Adrien  
Tibiletti, Tania
Caffarri, Stefano
Chergui, Majed  
Date Issued

2014

Publisher

American Institute of Physics

Published in
Review Of Scientific Instruments
Volume

85

Issue

10

Article Number

103118

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LSU  
Available on Infoscience
December 30, 2014
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/109823
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