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  4. Effect of antibiotics on mechanical properties of Bordetella pertussis examined by atomic force microscopy
 
research article

Effect of antibiotics on mechanical properties of Bordetella pertussis examined by atomic force microscopy

Villalba, M. I.  
•
Venturelli, L.  
•
Arnal, L.
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April 1, 2022
Micron

In recent years, the coevolution of microorganisms with current antibiotics has increased the mechanisms of bacterial resistance, generating a major health problem worldwide. Bordetella pertussis is a bacterium that causes whooping cough and is capable of adopting different states of virulence, i.e. virulent or avirulent states. In this study, we explored the nanomechanical properties of both virulent and avirulent B. pertussis as exposed to various antibiotics. The nanomechanical studies highlighted that only virulent B. pertussis cells undergo a decrease in their cell elastic modulus and height upon antimicrobial exposure, whereas their avirulent counterparts remain unaffected. This study also permitted to highlight different mechanical properties of individual cells as compared to those growing in close contact with other individuals. In addition, we analyzed the presence on the bacterial cell wall of Filamentous hemagglutinin adhesin (FHA), the major attachment factor produced by virulent Bordetella spp., under different virulence conditions by Force Spectroscopy.

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Type
research article
DOI
10.1016/j.micron.2022.103229
Web of Science ID

WOS:000783878500004

Author(s)
Villalba, M. I.  
Venturelli, L.  
Arnal, L.
Masson, C.
Dietler, G.  
Vela, M. E.
Yantorno, O.
Kasas, S.  
Date Issued

2022-04-01

Publisher

PERGAMON-ELSEVIER SCIENCE LTD

Published in
Micron
Volume

155

Article Number

103229

Subjects

Microscopy

•

Microscopy

•

afm

•

bordetella pertussis

•

virulence

•

antibiotics

•

escherichia-coli

•

filamentous hemagglutinin

•

nanomechanical properties

•

virulence regulon

•

unbinding forces

•

microbial-cells

•

high prevalence

•

bacteria

•

surface

•

adhesin

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LPMV  
Available on Infoscience
May 23, 2022
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/188063
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