Repository logo

Infoscience

  • English
  • French
Log In
Logo EPFL, École polytechnique fédérale de Lausanne

Infoscience

  • English
  • French
Log In
  1. Home
  2. Academic and Research Output
  3. Journal articles
  4. Submicrometer optical tomography by multiple-wavelength digital holographic microscopy
 
research article

Submicrometer optical tomography by multiple-wavelength digital holographic microscopy

Montfort, Frédéric  
•
Colomb, Tristan  
•
Charrière, Florian  
Show more
2006
Applied Optics

We present a method for submicrometer tomographic imaging using multiple wavelengths in digital holographic microscopy. This method is based on the recording, at different wavelengths equally separated in the k domain, in off- axis geometry, of the interference between a reference wave and an object wave reflected by a microscopic specimen and magnified by a microscope objective. A CCD camera records the holograms consecutively, which are then numerically reconstructed following the convolution formulation to obtain each corresponding complex object wavefront. Their relative phases are adjusted to be equal in a given plane of interest and the resulting complex wavefronts are summed. The result of this operation is a constructive addition of complex waves in the selected plane and destructive addition in the others. Tomography is thus obtained by the attenuation of the amplitude out of the plane of interest. Numerical variation of the plane of interest enables one to scan the object in depth. For the presented simulations and experiments, 20 wavelengths are used in the 480-700 nm range. The result is a sectioning of the object in slices 725 nm thick.

  • Details
  • Metrics
Type
research article
DOI
10.1364/AO.45.008209
Web of Science ID

WOS:000241888200006

Author(s)
Montfort, Frédéric  
Colomb, Tristan  
Charrière, Florian  
Kühn, Jonas
Marquet, Pierre
Cuche, Etienne  
Herminjard, Sylvain
Depeursinge, Christian  
Date Issued

2006

Published in
Applied Optics
Volume

45

Issue

32

Start page

8209

End page

8217

Subjects

[MVD]

•

Holography

•

Computer holography

•

Image processing

•

Image reconstruction techniques

•

Imaging systems

•

Microscopy

•

Three-dimensional image acquisition

•

Tomography

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LOA  
Available on Infoscience
July 20, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/41621
Logo EPFL, École polytechnique fédérale de Lausanne
  • Contact
  • infoscience@epfl.ch

  • Follow us on Facebook
  • Follow us on Instagram
  • Follow us on LinkedIn
  • Follow us on X
  • Follow us on Youtube
AccessibilityLegal noticePrivacy policyCookie settingsEnd User AgreementGet helpFeedback

Infoscience is a service managed and provided by the Library and IT Services of EPFL. © EPFL, tous droits réservés