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  4. Extended focus Fourier domain optical coherence microscopy assists developmental biology
 
conference paper

Extended focus Fourier domain optical coherence microscopy assists developmental biology

Villiger, Martin L.
•
Beleut, Manfred  
•
Brisken, Cathrin  
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2007
Optical Coherence Tomography And Coherence Techniques III
Optical Coherence Tomography and Coherence Techniques III

We present a novel detection scheme for Fourier domain optical coherence microscopy (FDOCM). A Bessel-like interference pattern with a strong central lobe was created with an axicon lens. This pattern was then imaged by a telescopic system into the sample space to obtain a laterally highly confined illumination needle, extending over a long axial range. For increased efficiency, the detection occurs decoupled from the illumination, avoiding a double pass through the axicon. Nearly constant transverse resolution of ~1.5µm along a focal range of 200µm with a maximum sensitivity of 105dB was obtained. A broad bandwidth Ti:Sapphire laser allowed for an axial resolution of 3µm in air, providing the nearly isotropic resolution necessary to access the microstructure of biological tissues. Together with the speed- and sensitivity-advantage of FDOCT, this system can perform in vivo measurements in a minimally invasive way. Tomograms of the mouse mammary gland and the mouse follicle, recorded in vitro, revealed biologically relevant structural details. Images acquired with classical microscopy techniques, involving stained and fluorescent samples, validate these structures and emphasize the high contrast of the tomograms. It is comparable to the contrast achieved with classical techniques, but employing neither staining, labeling nor slicing of the samples, stressing the high potential of FDOCM for minimally invasive in vivo small animal imaging.

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Type
conference paper
DOI
10.1117/12.728370
Web of Science ID

WOS:000251474900034

Author(s)
Villiger, Martin L.
Beleut, Manfred  
Brisken, Cathrin  
Lasser, Theo
Leitgeb, Rainer A.
Date Issued

2007

Publisher

SPIE

Published in
Optical Coherence Tomography And Coherence Techniques III
Series title/Series vol.

SPIE Proceedings; 6627

Start page

66271H

Editorial or Peer reviewed

NON-REVIEWED

Written at

EPFL

EPFL units
UPBRI  
LOB  
Event nameEvent placeEvent date
Optical Coherence Tomography and Coherence Techniques III

Munich, Germany

Sunday 17 June 2007

Available on Infoscience
July 3, 2008
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/26540
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