Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes
Nucleic acid-templated reactions leading to a fluorescent product represent an attractive strategy for the detection and imaging of cellular nucleic acids. Herein we report the use of a Staudinger reaction to promote the reduction of profluorescent azidorhodamine. The use of two cell-permeable GPNA probes, one labeled with the profluorescent azidorhodamine and the other with trialkylphosphine, enabled the detection of the mRNA encoding O-6-methylguanine-DNA methyltransferase in intact cells.
Cite this item
Pianowski, Z., Gorska, K., Oswald, L., Merten, C., & Winssinger, N. (2009). Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes. Journal of the American Chemical Society, 131(18), 6492–6497. https://doi.org/10.1021/ja809656k
Click Copy to copy the citation, or select the text manually.
2009-04-20
131
18
6492
6497
REVIEWED
OTHER