In this study, we propose the development and simulation of an electrochemical aptamer biosensor designed for point-of-care detection of tuberculosis (TB) in human urine. The biosensor operates through amperometric biosensing combined with aptamer-antibody sandwich assays using the TB-antigen CFP-10. Theoretical calculations conducted in this research demonstrate the potential for highly sensitive and accurate read-out, with a linear range of 0.17-0.53 nA, covering concentrations from 3 to 10 nM. Furthermore, the incorporation of an Slayer filter enables efficient sample filtration, enhancing the sensitivity and selectivity of the biosensor for detecting TB during urination events without the need for professional healthcare assistance. Therefore, this system is well-suited for deployment outside the laboratory, particularly in resource-limited settings or underdeveloped regions.