Pressing adhering live cells onto the nanostructured SiN substrate and subsequent removal, planar cell membrane sheets (CMSs) were transferred in a well-defined orientation onto the SiN support. Using perforated thin SiN membranes both, extracellular and intracellular surfaces of CMSs become simultaneously accessible by targeting membrane constituents side-specifically with fluorescent markers. The perforation of the membranes has been performed using FIB technique.