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research article

Microscopy image resolution improvement by deconvolution of complex fields

Cotte, Yann  
•
Toy, Muhammed Fatih  
•
Pavillon, Nicolas  
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2010
Optics Express

Based on truncated inverse filtering, a theory for deconvolution of complex fields is studied. The validity of the theory is verified by comparing with experimental data from digital holographic microscopy (DHM) using a high-NA system (NA=0.95). Comparison with standard intensity deconvolution reveals that only complex deconvolution deals correctly with coherent cross-talk. With improved image resolution, complex deconvolution is demonstrated to exceed the Rayleigh limit. Gain in resolution arises by accessing the objects complex field - containing the information encoded in the phase - and deconvolving it with the reconstructed complex transfer function (CTF). Synthetic (based on Debye theory modeled with experimental parameters of MO) and experimental amplitude point spread functions (APSF) are used for the CTF reconstruction and compared. Thus, the optical system used for microscopy is characterized quantitatively by its APSF. The role of noise is discussed in the context of complex field deconvolution. As further results, we demonstrate that complex deconvolution does not require any additional optics in the DHM setup while extending the limit of resolution with coherent illumination by a factor of at least 1.64.

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Type
research article
DOI
10.1364/OE.18.019462
Web of Science ID

WOS:000281779600003

Author(s)
Cotte, Yann  
Toy, Muhammed Fatih  
Pavillon, Nicolas  
Depeursinge, Christian  
Date Issued

2010

Publisher

Optical Society of America

Published in
Optics Express
Volume

18

Issue

19

Start page

19462

End page

19478

Subjects

[MVD]

•

Coherence and statistical optics : Coherent optical effects

•

Image processing : Deconvolution

•

Image processing : Phase retrieval

•

Image processing : Superresolution

•

Imaging systems : Microscopy

•

Holography : Digital holography

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LOA  
Available on Infoscience
August 27, 2010
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/52533
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