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  4. Optimization of concomitant production of cellulase and xylanase from Rhizopus oryzae SN5 through EVOP-factorial design technique and application in Sorghum Stover based bioethanol production
 
research article

Optimization of concomitant production of cellulase and xylanase from Rhizopus oryzae SN5 through EVOP-factorial design technique and application in Sorghum Stover based bioethanol production

Pandey, Ajay Kumar
•
Gnansounou, Edgard  
•
Negi, Sangeeta
2016
Renewable Energy

Current study deals with the production of cellulases and xylanases from the Rhizopus oryzae SN5 isolated from composed soil of Himalayan pine forest, in order to meet the challenges of lignocellulosic biomass based biorefineries. Culture parameters for concomitant production of cellulase and xylanase were optimized through EVOP-factorial design technique under solid state fermentation. And maximum yield of cellulase and xylanase were obtained 437.54 U/gds and 273.83 U/gds, respectively at 30 o C and pH 6.0 after 5 days of incubation. On applying these enzymes for the saccharification of the dilute acid pretreated Sorghum Stover (SS), 0.407 g/g sugar was yielded. This hydrolysate on fermentation, yielded 0.411 g/g ehanol with Saccharomyces cerevisiae (NCIM 3288), which could be considered a good conversion. Therefore, Rhizopus oryzae SN5 was found as potent strain for the production of the cocktail of lignocellulosic biomasss hydrolytic enzymes and would be promising tool in the area of lignocellulose based bio-refineries.

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Type
research article
DOI
10.1016/renene.2016.05.071
Author(s)
Pandey, Ajay Kumar
Gnansounou, Edgard  
Negi, Sangeeta
Date Issued

2016

Publisher

Pergamon-Elsevier Science Ltd

Published in
Renewable Energy
Issue

98

Start page

51

End page

56

Subjects

Cellulase

•

Xylanase

•

Saccharification

•

Ethanol

•

Rhizopus oryzae

Editorial or Peer reviewed

NON-REVIEWED

Written at

OTHER

EPFL units
GR-GN  
Available on Infoscience
October 28, 2016
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/130848
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