Light-field tomographic fluorescence lifetime imaging microscopy (LIFT-FLIM)
Fluorescence lifetime imaging microscopy (FLIM) measures fluorescence lifetimes of fluorescent probes to investigate molecular interactions. However, conventional FLIM systems often requires extensive scanning that is time-consuming. To address this challenge, we developed a novel computational imaging technique called light field tomographic FLIM (LIFT-FLIM). Our approach acquires volumetric fluorescence lifetime images in a highly data-efficient manner, significantly reducing the number of scanning steps. We demonstrated LIFT-FLIM using a single-photon avalanche diode array on various biological systems. Additionally, we expanded to spectral FLIM and demonstrated high-content multiplexed imaging of lung organoids. LIFT-FLIM can open new avenues in the biomedical research.
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Ma, Y., Huang, L., Sen, C., Burri, S., Bruschini, C., Yang, X., Cameron, R. B., Fishbein, G. A., Gomperts, B. N., Özcan, A., Charbon, E., & Gao, L. (2024). Light-field tomographic fluorescence lifetime imaging microscopy (LIFT-FLIM). In L. Gao, G. Zheng, & S. A. Lee (Editors), Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences. Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences, San Francisco, United States, January 27, 2024. https://doi.org/10.1117/12.3002375
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University of California, Los Angeles
University of California, Los Angeles
University of California, Los Angeles
École Polytechnique Fédérale de Lausanne
EPFL
University of California, Los Angeles
University of California, Los Angeles
University of California, Los Angeles
University of California, Los Angeles
University of California, Los Angeles
2024-03-13
Proceedings SPIE; PC12857
46
REVIEWED
EPFL
| Event name | Event acronym | Event place | Event date |
San Francisco, United States | 2024-01-27 - 2024-02-01 | ||