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research article

DNA as a Recyclable Natural Polymer

Liu, Weina  
•
Giaveri, Simone  
•
Ortiz, Daniel  
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February 15, 2022
Advanced Functional Materials

Nature has the ability of circularly re-using its components to produce the molecules and materials it needs. An example is the ability of most living organisms of digesting proteins they feed off into amino acids and then using such amino acids in the ribosomal synthesis of new proteins. Recently, it has been shown that such recycling of proteins can be reproduced outside living organisms. The key proteins' feature that allows for this type of recycling is their being sequence-defined polymers. Arguably, nature's most famous sequence-defined polymer is DNA. Here it is shown that it is possible, starting from sheared calf-DNA, to obtain all four nucleotides as monophosphate-nucleotides (dNMPs). These dNMPs are phosphorylated in a one-pot, multi-enzymes, phosphorylation reaction to generate triphosphate-nucleotides (dNTPs). Finally, dNTPs so achieved (with a global yield of similar to 60%) are used as reagents for PCR (polymerase chain reaction) to produce target DNA strands, and for the diagnosis of targeted DNA by quantitative PCR (qPCR). This approach is an efficient, convenient, and environmentally friendly way to produce dNTPs and DNA through recycling according to the paradigm of a circular economy.

  • Details
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Type
research article
DOI
10.1002/adfm.202109538
Web of Science ID

WOS:000755635700001

Author(s)
Liu, Weina  
Giaveri, Simone  
Ortiz, Daniel  
Stellacci, Francesco  
Date Issued

2022-02-15

Publisher

WILEY-V C H VERLAG GMBH

Published in
Advanced Functional Materials
Article Number

2109538

Subjects

Chemistry, Multidisciplinary

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Chemistry, Physical

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Nanoscience & Nanotechnology

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Materials Science, Multidisciplinary

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Physics, Applied

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Physics, Condensed Matter

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Chemistry

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Science & Technology - Other Topics

•

Materials Science

•

Physics

•

dna hydrolysis

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dna recycling

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one-pot phosphorylation

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qpcr

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escherichia-coli

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cell lysate

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nucleoside

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nucleotides

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methylation

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kinase

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
SUNMIL  
Available on Infoscience
March 14, 2022
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/186271
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