An increase in urban vegetation is an often proposed mitigation strategy to reduce urban heat and improve outdoor thermal comfort (OTC). Vegetation can alter urban microclimate through changes in air temperature, mean radiant temperature, humidity, and wind speed. In this study, we model how street tree and ground vegetation cover and their structural, optical, interception, and physiological traits control the diurnal cycle of OTC in different urban densities in a tropical city (Singapore). For this purpose, we perform a variance based sensitivity analysis of the urban ecohydrological model UT&C. Model performance is evaluated through a comparison with local microclimate measurements and OTC is assessed with the Universal Thermal Climate Index (UTCI). We find a pronounced daily cycle of vegetation effects on UTCI. Tree cover fraction is more efficient in decreasing UTCI during daytime, while a higher vegetated ground fraction provides more cooling during night. Generally, increasing vegetation cover fractions do not deter OTC, except in certain urban densities during some periods of the day. An increase in tree and ground vegetation fractions provides a higher average UTCI reduction compared to a change in vegetation traits (0.9 – 2.9 °C vs. 0.7 – 1.1 °C during midday, 10 month average). The increase in humidity related to plant transpiration prevents further reduction of UTCI. However, the choice of vegetation traits enhancing tree transpiration can decrease UTCI during hot periods. These results can inform urban planners on the selection of vegetation amount and traits to achieve feasible OTC improvements in tropical cities.